SE Minnesota Emergency Medical Services (EMS) agencies are Coalition partners through a Joint Powers Board organization, Southeastern Minnesota Emergency Medical Services, which includes the following agencies.
| Adams Area Ambulance | Mayo Clinic Ambulance Albert Lea |
| Altura Ambulance | Mayo Clinic Ambulance Austin |
| Blooming Prairie Ambulance | Mayo Clinic Ambulance Owatonna |
| Caledonia Ambulance | Mayo Clinic Ambulance Rochester |
| Cannon Falls Ambulance | Mayo One |
| Chatfield Ambulance | North Memorial Faribault |
| Dodge Center Ambulance | Northfield Fire Rescue |
| Elgin Ambulance | Plainview EMS |
| Ellendale Ambulance | Preston Emergency Service |
| Eyota Ambulance | Red Wing Fire Department |
| Freeborn Fire Department & Ambulance | Rushford Community Ambulance |
| Grand Meadow Area Ambulance | Spring Grove Ambulance |
| Harmony Volunteer Ambulance | Spring Valley Area Ambulance |
| Hayfield Community Ambulance | St. Charles Ambulance |
| Houston Community Ambulance | Tri-State Ambulance |
| Lake City Ambulance | Wabasha Ambulance |
| Lanesboro Ambulance | West Concord Fire Dept. Ambulance |
| Le Roy Area Ambulance | Winona Area Ambulance |
| Lewiston Ambulance | Zumbrota Area Ambulance |
| Mabel Ambulance | |
As fiscal agent for EMS agencies, Lewiston Ambulance is a direct Coalition partner signatory.
